Accutase®
Cell Detachment Solution
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Accutase in ActionSemper Lenis "Always Gentle" reattachment of neuronal stem cells passaged with Accutase.
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"I like that Accutase is gentle on cells and auto-inhibits at 37°C without the need for a neutralizing solution like with trypsin. Also, the cost of Accutase is less than trypsin + trypsin neutralizing solution, so it is cost-effective. It is also simpler to use, because it doesn't require the extra neutralizing step, I just lift my cells in Accutase, then add them directly into fresh media.
I believe everyone in my lab now uses Accutase. We work with a wide range of cancer cells and neuronal cells too, and it works great."
- Jennifer Smith Ph.D, Postdoctorial Researcher, UCSB
Accutase® is a ready to use non-mammalian, non-bacterial replacement for all applications of trypsin.
Accutase is a natural enzyme mixture with proteolytic and collagenolytic enzyme activity. This means it mimics the action of trypsin and collagenase at the same time. However, because it is more efficient than mammalian trypsin & collagenase, it is formulated at a much lower concentration making it less toxic and gentler, but just as effective.
Advantages of Accutase
Cell Lines tested
A few cell lines that Accutase has been shown to detach without harm:
hESCs, fibroblasts, keratinocytes, vascular endothelial cells, hepatocytes, vascular smooth muscle cells, hepatocyte progenitors, primary chick embryo neuronal cells, bone marrow stem cells, adherent CHO and BHK cells, macrophages, 293 cells, L929 cells, immortalized mouse testicular germ cells, 3T3, Vero, COS, HeLa, NT2, MG63, M24 and A375 metastatic melanoma, gliomas U251 and D54, HT1080 fibrosarcoma
cells, and Sf9 insect cells.
Applications
Accutase performs exceptionally well in detaching cells for:
hESC culturing, analysis of cell surface markers, virus growth assay, quiescence assays by serum starvation, transformation assays by oncogene transfection, neural crest cell migration assays, cell proliferation, apoptosis, cell haptotaxsis, tumor cell migration assays, routine cell passage, production scale-up (bioreactor), and flow cytometry.
Accutase is a natural enzyme mixture with proteolytic and collagenolytic enzyme activity. This means it mimics the action of trypsin and collagenase at the same time. However, because it is more efficient than mammalian trypsin & collagenase, it is formulated at a much lower concentration making it less toxic and gentler, but just as effective.
Advantages of Accutase
- Can be used whenever gentle and efficient detachment of any adherent cell line is needed. Accutase is a direct replacement for trypsin.
- Works extremely well on embryonic and neuronal stem cells; mono layers of stem cells can be grown after passaging with Accutase.
- Preserves most epitopes for subsequent flow cytometry analysis.
- Does not need to be neutralized when passaging adherent cells. The addition of more media after the cells are split dilutes Accutase so it is no longer able to detach cells.
- Does not need to be aliquoted. A bottle is stable in the refrigerator for 2 months.
Cell Lines tested
A few cell lines that Accutase has been shown to detach without harm:
hESCs, fibroblasts, keratinocytes, vascular endothelial cells, hepatocytes, vascular smooth muscle cells, hepatocyte progenitors, primary chick embryo neuronal cells, bone marrow stem cells, adherent CHO and BHK cells, macrophages, 293 cells, L929 cells, immortalized mouse testicular germ cells, 3T3, Vero, COS, HeLa, NT2, MG63, M24 and A375 metastatic melanoma, gliomas U251 and D54, HT1080 fibrosarcoma
cells, and Sf9 insect cells.
Applications
Accutase performs exceptionally well in detaching cells for:
hESC culturing, analysis of cell surface markers, virus growth assay, quiescence assays by serum starvation, transformation assays by oncogene transfection, neural crest cell migration assays, cell proliferation, apoptosis, cell haptotaxsis, tumor cell migration assays, routine cell passage, production scale-up (bioreactor), and flow cytometry.
Questions? Call 858.587.1716 or
Check out our FAQ Page!
Pricing
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Accutase - 100 ml - $30 each
Cat# AT104 |
Accutase - 500 ml - $130 each
Cat# AT104-500 |
Cell Detachment
Human MG63 Osteosarcoma cells cultured in DMEM + 10% FBS were treated with Accutase. Treatment resulted in rapid cell detachment, a single cell suspension, and high viability. Accutase is gentle on cells; viability was 97 ± 3% even after 45 minutes in Accutase. |
Accutase Protocols |
Dissolving Neurospheres?
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