Accumax
Cell Dissociation Solution
"In our Neuro-Oncology lab, we grow primary cultures of tumor cells generated from tissue samples collected at surgical resection. Brain tumors are notoriously heterogeneous with multiple cell types. Harvesting our cultures with Accumax ensures that we are able to remove all the cells from the flasks so that we don't 'lose' subtypes of cells that adhere more tightly to the plastic, while maintaining cell viability, so that our in vitro cell cultures mimic the biological properties of the original tumor specimens."
- Sharon K. Michelhaugh, PhD, Assistant Profession (Research), Wayne State University School of Medicine
Accumax Cell Dissociation Solution
Accumax is a ready to use non-mammalian, non-bacterial replacement for all applications of trypsin and collagenase in tissue dissociation, cell counting, and dissolving cell clumps such as spheroids.
Accumax contains the same enzymes as Accutase and is a direct replacement for collagenase.
Accumax can be used to increase the accuracy of a cell count. It can also be added to a clumpy sample on a cell sorter to extend the sort time of the sample.
Advantages of Accumax
Cell Lines tested
A few cell lines that Accumax has been shown to work on without harm:
hESCs, fibroblasts, keratinocytes, vascular endothelial cells, hepatocytes, vascular smooth muscle cells, hepatocyte progenitors, primary chick embryo neuronal cells, bone marrow stem cells, adherent CHO and BHK cells, macrophages, 293 cells, L929 cells, immortalized mouse testicular germ cells, 3T3, Vero, COS, HeLa, NT2, MG63, M24 and A375 metastatic melanoma, gliomas U251 and D54, HT1080 fibrosarcoma cells, and Sf9 insect cells.
Applications
Accumax performs exceptionally well for:
hESC culturings, analysis of cell surface markers, virus growth assay, quiescence assays by serum starvation, transformation assays by oncogenetransfection, neural crest cell migration assays, cell proliferation, apoptosis, cell haptotaxsis, tumor cell migration assays, routine cell passage, production scale-up (bioreactor), and flow cytometry. Accumax is used to digest primary tissue, create single cell suspensions for cell counts, and declump cells for magnetic or flow cytometry cell sorting and removing from artificial growing matrixes.
Accumax contains the same enzymes as Accutase and is a direct replacement for collagenase.
Accumax can be used to increase the accuracy of a cell count. It can also be added to a clumpy sample on a cell sorter to extend the sort time of the sample.
Advantages of Accumax
- Dissociates tissue.
- Increases accuracy of manual or automated cell counts.
- Used to dissolve neuronal and prostate spheroids.
- Removes cells from 3-D matrixes.
- Removes cells from hollow fiber cell reactors.
- Extends the sort time of clumpy cell on a sorting flow cytometer.
Cell Lines tested
A few cell lines that Accumax has been shown to work on without harm:
hESCs, fibroblasts, keratinocytes, vascular endothelial cells, hepatocytes, vascular smooth muscle cells, hepatocyte progenitors, primary chick embryo neuronal cells, bone marrow stem cells, adherent CHO and BHK cells, macrophages, 293 cells, L929 cells, immortalized mouse testicular germ cells, 3T3, Vero, COS, HeLa, NT2, MG63, M24 and A375 metastatic melanoma, gliomas U251 and D54, HT1080 fibrosarcoma cells, and Sf9 insect cells.
Applications
Accumax performs exceptionally well for:
hESC culturings, analysis of cell surface markers, virus growth assay, quiescence assays by serum starvation, transformation assays by oncogenetransfection, neural crest cell migration assays, cell proliferation, apoptosis, cell haptotaxsis, tumor cell migration assays, routine cell passage, production scale-up (bioreactor), and flow cytometry. Accumax is used to digest primary tissue, create single cell suspensions for cell counts, and declump cells for magnetic or flow cytometry cell sorting and removing from artificial growing matrixes.
Increase in Cell Counts
Various constructs of genetically engineered CHO cells, BHK cells, and a hybridoma were grown in suspension in serum-free or protein-free medium.
Representative cell aliquots were treated with an equal volume of PBS or ACCUMAX and incubated for 5 minutes at 37C. Cell number was then determined with a Coulter Counter.
Sorting cells on a flow cytometer?
|
|
